Gram Positive Bacteria Stain This Color Under The Microscope

Why Do Bacteria Stain Differently After Gram Stain?

Web stains, or dyes, contain salts made up of a positive ion and a negative ion. Web the gram stain procedure distinguishes between gram positive and gram negative groups by coloring these cells red or violet. Depending on the type of dye, the positive or the negative ion may be the chromophore (the colored ion);

In Their Natural State, Most Of The Cells And Microorganisms That We Observe Under The Microscope Lack Color And Contrast.

Gram positive cells remain purple; (top) microscopic images of bacteria stained with the gram stain. Web introduction of gram stain.

Gram Negative Cells Are Now Pink/Red :

Red color examples gram positive bacteria: What are the typical dimensions of pseudomonas aeruginosa cells when viewed under high magnification? When washed with solvent, the cell pores close becoming less permeable and are able to retain the stain, in this case, purple.

Some Stains Commonly Used For Simple Staining Include Crystal Violet, Safranin, And Methylene Blue.

Web gram positive bacteria retain the crystal violet even through the decolorizor step: Web describe the unique features of commonly used stains. Other information used to differentiate bacteria is the shape.

The Key Step To Their Differentiation Is The Decolorization.

The theory of gram stain differentiation is based on cell wall structure and lipid component; Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with. Actinomyces, bacillus, clostridium, corynebacterium, enterococcus, gardnerella, lactobacillus, listeria, mycoplasma, nocardia, staphylococcus, streptococcus, streptomyces ,etc.